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Light Sheet Microscope

源自EMBL实验室的原创Lightsheet技?/span>

MuVi-SPIM

IMAGING LIFE IN THE FAST LANE

Latest sCMOS camera technology and optimized combinations of objective lenses enable you to obtain the best from your sample: data you always wanted but could never get.

  • Intrinsic 3D optical sectioning

  • Large field of view

  • Close-to-confocal resolution

  • Very high imaging speed

  • Highest sensitivity and minimal noise

360 ILLUMINATION AND DETECTIONThe four objective lenses allow four combinations of illumination and detection. This provides four orthogonal views of the specimen without the need for sample rotation enabling:

  • Multiple view imaging of large samples at unprecedented speed

  • Long-term stability

  • Time series over several days

  • Robust image registration

  • Efficient data fusion and visualization

Laser

  • Laser combiner with six laser positions

  • Available laser lines 445 488 515 532 561 594 642 and 685 nm with 50 mW each

  • Fast modulation and high extinction
Illumination optics

  • Two identical illumination arms

  • Chromatic correction from 440 to 660 nm

  • Light-sheet generation by beam scanning

  • Variable light-sheet thickness (optional)

  • Water-dipping objective lenses (see below)
Detection optics

  • Two identical detection arms

  • Water-dipping objective lenses (see below)

  • Fast filter wheels with 10 positions and 50 ms between adjacent positions

  • Filters adapted to the selected laser lines

  • Variable magnification (optional)

  • High-speed sCMOS cameras Hamamatsu Orca Flash 4.0 with 2048 x 2048 pixels of 6.5 m x 6.5 m size
Objective lens mounting unit

  • High precision mounting unit

  • Fast exchange of the unit with high precision

  • Mounting chamber with temperature control (see below)

  • 2 x Nikon CFI Plan Fluor 10XW 0.3 NA water immersion for illumination

  • 2 x Nikon CFI Apo LWD 25XW 1.1 NA water immersion for detection

  • Other objective lenses on request
Sample chamber and stage

  • Water-sealed inert PEEK plastic chamber autoclavable and biocompatible

  • Sample supported from below for improved stability

  • Easy access from above for sample mounting injections etc.

  • Fast and precise temperature control range 15-40C

  • High-precision XYZ and fast rotation stage

  • XYZ piezo crawler stage with 100 nm resolution and 8.5 mm (X) and 1.2 mm (YZ) travel range for fast sample positioning and stack acquisition

  • Custom-made rotation stage with > 360 degrees/second speed
Electronics Computer Software

  • All electronic components included in the microscope housing

  • Embedded microscope software with open communication interface

  • Open GUI control for interactive control and microscope automation

  • Computer with 64 GB RAM Intel Dual Six Core CPU

  • High-speed RAID controller for data streaming

  • 8 x 4 TB local storage in RAID 0

  • One 40 inch monitor
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