FL3500/F快速版叶绿素荧光仪

FL 3500/F快速版叶绿素荧光仪皃�/span>时间分辨率在标准版的基础上提高到1 ��s，可精确捕捉和描绘快速荧光瞬变过程，使得运用PAM技术对OIJP曲线和光闪荧光诱导的快速动力学测量更加准确高效，能够更加精确地计算各种相关参数、�/span>

该系统可用于蓝绿藻或绿藻等微藻，叶绿体或类囊体悬浮物，乃至叶片的光合作用研究的强大科研工具。其核心结构是包含了一个悬浮液标准样品杯的光学测量头，内置3练�/span>LED光源咋�/span>1�?/span>500 kHz/16佌�/span>AD转换皃�/span>PIN二极管信号检测器、�/span>AD转换的增益和积分时间可以通过软件控制。检测器测量叶绿素荧光信号的时间分辨率可高达1 ��s。系统配置的光合放氧测量模块，使其可以对放氧复合物的状态转换做更深入研究、�/span>

应用领域９�/span>

��植物光合特性和代谢紊乱筛逈�/span>

��生物和非生物胁迫的检浊�/span>

��植物抗胁迫能力或者易感性研穵�/span>

��代谢混乱研究

��光合系统工作机理研究

��受胁迫植物光合生理应对策略研穵�/span>

典型样品９�/span>

��蓝藻（蓝细菌（�/span>

��绿藻

��叶绿体悬浮物

��类囊体悬浮物

��植物碎片

功能特点９�/span>

��具备双通道测量控制，可以与各种测量单元如标准版、快速版、叶夹式及水下测量头等配合使用，各版本的测量头可互换使用

��内置多种可用户自行修改的测量程序，涵盖目前国际上对于叶绿素荧光的各种一般性研究和深入机理研究

��配备单翻转光＇�/span>STF），可进衋�/span>QA‒�/span>再氧化动力学�?/span>S状态转换等光合机理研究

��配备高灵敏度检测器，可进行OJIP快速荧光动力学分析

��可控制测量样品的温度并进行磁力搅拋�/span>

技术参数：

��实验程序：叶绿素荧光诱导测量：�/span>PAM（脉冲调制）测量：�/span>OJIP快速荧光动力学测量：�/span>QA‒�/span>再氧化动力学：�/span>S状态转换；叶绿素荧光淬灭；光系绞�/span>II有效天线色素大小

• 荧光参数９�/span>F₀＋�/span>Fm＋�/span>Fv＋�/span>F₀‘�span>＋�/span>Fm‘�span>＋�/span>Fv‘�span>＋�/span>QY(II)＋�/span>NPQ＋�/span>��PSII＋�/span>Fv/Fm＋�/span>Fv�?Fm‘�span>＋�/span>Rfd＋�/span>qN＋�/span>qP＋�/span>ETR筈�/span>50多项叶绿素荧光参数与图像＋�/span>OJIP曲线不�/span>F₀�?/span>FJ�?/span>Fi�?/span>Fm�?/span>Fv�?/span>VJ�?/span>Vi�?/span>Fm / F₀�?/span>Fv / F₀�?/span>Fv / Fm�?/span>M0�?/span>Area�?/span>Fix Area�?/span>SM�?/span>SS�?/span>N�?/span>Phi_P₀�?/span>Psi_₀�?/span>Phi_E₀�?/span>Phi_D₀�?/span>Phi_Pav�?/span>ABS / RC�?/span>TR₀/ RC�?/span>ET₀/ RC�?/span>DI₀/ RC筈�/span>20多项相关参数

��时间分辨率（采样频率）：高灵敏度检测器，时间分辨率辽�/span>4��s

��控制单元：双通道通用高度精确性自动微处理器，可以与各种测量单元如标准版、快速版、叶夹式及水下测量头等配合使用（无需另行购买控制单元（�/span>

��测量光闪：标准为波长617nm的橙光或455nm的蓝光，光闪时间2‒�/span>5��s

��单翻转饱和光闪：标准光源丹�/span>630nm的红光，光闪时间20‒�/span>50��s

��持续光化学光：标准为630nm的红光或455nm的蓝光，**光强2500 ��mol(photons)/m2.s

��远红外光源（选配）：用于激发光系统I，波镾�/span>735nm

��氧电极（选配）：测量藻类的氧气释攽�/span>

��每组lED光源强度和时间可通关软件调控，同时光源可根据研究要求选配

��温度控制９�/span>TR 2000温度调节器，控温范围0‒�/span>70ℂ�/span>，精确度0.1ℂ�/span>

��电磁搅拌９�/span>8mm��3mm标准磁力棑�/span>

��样品试管：底面积10��10mm，容�?/span>4ml

��AD转换器：试管式为500 kHz/16‒�/span>bit

��FluorWin软件：定义或创建实验方案、光源控制设置、数据输出、分析处理和图表显示

典型应用：荧光诱导过程分枏�/span>

产地：捷兊�/span>

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��Developmental Defects in Mutants of the PsbP Domain Protein5 inArabidopsis thaliana. JL Roose� et al� 2011. PloS one

��Inhibition of photosystems I and II activities in salt stress–exposed Fenugreek (Trigonella foenum graecum). M Zaghdoudi� et al� 2011. Journal of Photochemistry and Photobiology B: Biology

��Productivity correlated to photobiochemical performance of Chlorella mass cultures grown outdoors in thin–layer cascades. J Masoj��dek� et al� 2011. Journal of Industrial Microbiology & Biotechnology

��Binding Stoichiometry and Affinity of the Manganese–Stabilizing Protein Affects Redox Reactions on the Oxidizing Side of Photosystem II. JL Roose� et al� 2011. Biochemistry