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MAVEn?高通量16通道果蝇代谢监测系统

果蝇作为经济实用的模式动物,可用于中枢神经系统紊乱、炎症性病变、心血管疾病、癌症以及糖尿病等治疗研究,而这些疾病的发生从生理上来说都与生物个体长期的代谢功能异常密切相关、/span>

MAVEn?高通量16通道果蝇代谢监测系统是由世界知名的美国Sable Systems International动物代谢测量公司生产的一?6通道、高分辨率及自动化的果蝇代谢监测仪器,可广泛用于代谢紊乱造成的各种流行疾病治疗的机理研究、/span>

MAVEn?果蝇代谢系统作为果蝇代谢分型监测方面的权威产品,主要具备以下特点9/span>

1. 改变了传统的单只果蝇的封闭或半封闭式测量模式,实现每个测量室都有实时氓/span>

流通过的完全开放式测量,避免了测量时内出现缺氧(hypoxia)或高碳酸血痆/span>

(hypercapnia),可一次测量多?6只个体、/span>

2. 15秒就可以完成一只果蝇的代谢监测,这代表了目前技术的**水平、/span>

3. 数据可以通过SD卡把带时间标签的CSV格式直接导出到电脑、/span>

4. 可选配FLIC果蝇觅食、AD-2果蝇活动、气体(氧气、二氧化碳、水汽以及其它可

检测气体)等监测单元、/span>

5. 参考文?多,高达4万多篇,属于前沿科技、/span>

具体性能指标9/span>

1. 气流流速:5毫升/分钟-200毫升/分钟,质量流量计,PID精确控制,精度为2%、/span>

2. 昆虫测量时间?5?3小时可程序化选择;基线测量时间:15?3小时可程庎/span>

化选择、/span>

3. 气压测量:分辨率1Pa,精?.05%、/span>

4. 光照水平?.1-5000勒克斯、/span>

5. 温度测量?-50℃,分辨?.01℃,精度0.25℃、/span>

6. 模拟输入?个模拟输入,16bit分辨率,-5?5伏电压信号,可接SSI其它仪器

或实验室其它气体分析仪等、/span>

7. 数据格式:CSV格式;数据存储:SD卡,**支持32G的SD卡、/span>

8. 双通道高精度差分式氧气分析测量仪:测量技术:燃料电池原理氧气传感器,

双通道;氧气浓度量?-100%(用户可自定义设?个级别);差值量?#177;50%:/span>

精度0.1%(O2浓度2-100%时);分辨率0.0001%O2;漂秺 0.01%每小时(温度

恒定情况下);响应时间小?秒;24小时漂移<0.01%?0分钟噪音<3ppm RMS:/span>

数字过滤(噪音)0-40秒可调,增幅0.2秒,内置A/D转换器分辨率16bits;温

度、压力补偿;传感器温度测量范?-60℃,精度0.2℃,分辨?.001℃;?/span>

气压测量分辨?.0001kPa,精度为满量程的0.05%;适用流量范围5-2000mL/

min?通道模拟信号输出?-5V BNC)可输出通道1的氧气浓度,通道2的氧氓/span>

浓度??的差值,大气压;数字输出:RS-232;具4行文字LCD显示屏,带背

光,可同时显?个通道的氧气含量和它们的差值,以及大气压;独具PID

(Proportional-Integral-Derivative)温控单元,保证内部氧气传感器温度恒

定,进一步提高了氧气测量的精度和稳定性;供电12-24VDC?A,配交流电送/span>

配器;工作温度:5-45℃,无冷凝;重量6.4kg;尺?3.2cm35.6cm20.3cm

9. 超高精度二氧化碳分析测量仪:用于测量微小昆虫(比如果蝇、蚊子等)或蜰/span>

螨类微小动物的呼吸代谢,可同时测量CO2浓度和H2O浓度;CO2量程0-3000ppm:/span>

准确?1%;分辨率0.01ppm;H2O量程0-60mmol/mol;准确度1%:/span>

10. 二次抽样单元:内置气泵、精密针阀、质量流量计,可用来给气流样本做二次

抽样,也可单独作为气源使用;流量范围5-2000mL/min;精度为读数?0%:/span>

分辨?mL/min;具?行显示LCD显示屏;?-5V BNC模拟信号输出;数字输

出RS-232;供?2-15VDC?0-350mA,配交流电适配器;工作温度?-50℃,

无冷凝;重量1.5kg;尺?6cm13cm20cm:/span>

产地:美囼/span>

文献案例9/span>

?/span>2016年已发表的果蝇有关文献中,使?/span>SSI果蝇代谢监测系统的达14篇,2015平/span>11篇,截止目前相关文献共计500多篇、/span>

1.Andrew N R Ghaedi B Groenewald B. The role of nest surface temperatures and the brain in influencing ant metabolic rates[J]. Journal of Thermal Biology 2016 60: 132- 139.

2.Baaren J Dufour C M S Pierre J S et al. Evolution of life‏/span>history traits and mating strategy in males: a case study on two populations of a Drosophila parasitoid[J]. Biological Journal of the Linnean Society 2016 117(2): 231-240.

3.Bartholomew N R Burdett J M VandenBrooks J M et al. Impaired climbing and flight behaviour in Drosophila melanogaster following carbon dioxide anaesthesia[J]. Scientific reports 2015 5.

4.Basson C H Clusella-Trullas S. The behavior-physiology nexus: behavioral and physiological compensation are relied on to different extents between seasons[J]. Physiological and Biochemical Zoology 2015 88(4): 384-394.

5.Bosco G Clamer M Messulam E et al. EFFECTS OF OXYGEN CONCENTRATION AND PRESSURE ON Drosophila melanogaster: OXIDATIVE STRESS MITOCHONDRIAL ACTIVITY AND SURVIVORSHIP[J]. Archives of insect biochemistry and physiology 2015 88(4): 222-234.

6.Casas J Body M Gutzwiller F et al. Increasing metabolic rate despite declining body weight in an adult parasitoid wasp[J]. Journal of insect physiology 2015 79: 27-35.

7.Correa Y D C G Faroni L R A Haddi K et al. Locomotory and physiological responses induced by clove and cinnamon essential oils in the maize weevil Sitophilus zeamais[J]. Pesticide biochemistry and physiology 2015 125: 31-37.

8.DeVries Z C Kells S A Appel A G. Estimating the critical thermal maximum (CT max) of bed bugs Cimex lectularius: Comparing thermolimit respirometry with traditional visual methods[J]. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 2016 197: 52-57.

9.Dreiss A N Schaud R Bziers P et al. Social huddling and physiological thermoregulation are related to melanism in the nocturnal barn owl[J]. Oecologia 2016 180(2): 371-381.

10.Duun Rohde P Krag K Loeschcke V et al. A Quantitative Genomic Approach for Analysis of Fitness and Stress Related Traits in a Drosophila melanogaster Model Population[J]. International Journal of Genomics 2016 2016.

11.Fischer K E Gelfond J A L Soto V Y et al. Health effects of long-term rapamycin treatment: the impact on mouse health of enteric rapamycin treatment from four months of age throughout life[J]. PloS one 2015 10(5): e0126644.

12.Groom D J E Toledo M C B Welch K C. Wingbeat kinematics and energetics during weightlifting in hovering hummingbirds across an elevational gradient[J]. Journal of Comparative Physiology B 2016: 1-18.

13.Gudowska A Boardman L Terblanche J S. The closed spiracle phase of discontinuous gas exchange predicts diving duration in the grasshopper Paracinema tricolor[J]. Journal of Experimental Biology 2016: jeb. 135129.

14.Haddi K Mendes M V Barcellos M S et al. Sexual Success after Stress? Imidacloprid- Induced Hormesis in Males of the Neotropical Stink Bug Euschistus heros[J]. PloS one 2016 11(6): e0156616.

15.Haddi K Oliveira E E Faroni L R A et al. Sublethal exposure to clove and cinnamon essential oils induces hormetic-like responses and disturbs behavioral and respiratory responses in Sitophilus zeamais (Coleoptera: Curculionidae)[J]. Journal of economic entomology 2015: tov255.

16.Horvthov T Antol A Czarnoleski M et al. Does temperature and oxygen affect duration of intramarsupial development and juvenile growth in the terrestrial isopod Porcellio scaber (Crustacea Malacostraca)?[J]. ZooKeys 2015 (515): 67.

17.Kivel? S M Lehmann P Gotthard K. Do respiratory limitations affect metabolism of insect larvae before moulting: an empirical test at the individual level[J]. Journal of Experimental Biology 2016: jeb. 140442.

18.Lebeau J Wesselingh R A Van Dyck H. Nectar resource limitation affects butterfly flight performance and metabolism differently in intensive and extensive agricultural landscapes[C]//Proc. R. Soc. B. The Royal Society 2016 283(1830): 20160455.

19.MacMillan H A Schou M F Kristensen T N et al. Preservation of potassium balance is strongly associated with insect cold tolerance in the field: a seasonal study of Drosophila subobscura[J]. Biology letters 2016 12(5): 20160123.

20.Meyers P J Powell T H Q Walden K K O et al. Divergence of the diapause transcriptome in apple maggot flies: winter regulation and post-winter transcriptional repression[J]. Journal of Experimental Biology 2016: jeb. 140566.

21.Plav?in I Sta?kov T ?ery M et al. Hormonal enhancement of insecticide efficacy in Tribolium castaneum: Oxidative stress and metabolic aspects[J]. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology 2015 170: 19-27.

22.Rodrigues C G Krger A P Barbosa W F et al. Leaf Fertilizers Affect Survival and Behavior of the Neotropical Stingless Bee Friesella schrottkyi (Meliponini: Apidae: Hymenoptera)[J]. Journal of economic entomology 2016 109(3): 1001-1008.

23.Thienel M Canals M Bozinovic F et al. The effects of temperature on the gas exchange cycle in Agathemera crassa[J]. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 2015 183: 126-130.

24.Williams C M Chick W D Sinclair B J. A cross‏/span>seasonal perspective on local adaptation: metabolic plasticity mediates responses to winter in a thermal‏/span>generalist moth[J]. Functional Ecology 2015 29(4): 549-561.

25.Williams C M Szejner-Sigal A Morgan T J et al. Adaptation to Low Temperature Exposure Increases Metabolic Rates Independently of Growth Rates[J]. Integrative and comparative biology 2016: icw009.

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