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SM9000光谱仪是一种高分辨率光纤光谱测量仪,测量范围涵盖紫外光、可见光乃至近红外波段、/span>SM9000既可以单独使用,也可以与FKM多光谱荧光动态显微成像系统?/span>FL3500双调制叶绿素荧光仪等仪器联用,测量各种荧光的光谱组成。由于其具备超高的灵敏度,甚至可以测量单个细胞激发荧光的光谱。每秒可记录100练/span>16bits分辨率的光谱数据、/span>
功能特点9/span>
超高灵敏度,可检测单个细胞的荧光光谱
超高分辨率,可检浊/span>10s - 10ms的闪先/span>
采集频率辽/span>100欠/span>/秒,可检测动态光谰/span>
积分时间仍/span>1毫秒到数分钟可调
模块化设计,小巧耐用,热稳定性高
产热量极位/span>
技术参数:
光谱范围9/span>200 - 980nm
分辨率:可检浊/span>10s - 10ms的闪先/span>
采集频率9/span>100欠/span>/科/span>
积分时间9/span>1毫秒到数分钟可调
光学入口:直徃/span>0.5,数值孔径(NA(/span>=0.22,可拆卸SMA接头
入射狭缝9/span>70m1400m
光栅:平场型校正
波长**精确度:< 0.5nm
再现性:< 0.1nm
温度漂移9/span>< 0.01nm/K
像素光谱距离9/span>0.8nm
FWHM半高宽:3 - 4nm
杂散光:0.1%(氙?/span>340nm测量(/span>
CCD阵列像素数:104464
像素尺寸9/span>2424mm2
系统数据9/span>16Bit模数转换
可联用仪器:FKM多光谱荧光动态显微成像系统?/span>FL3500双调制叶绿素荧光仪等
不/span>FKM多光谱荧光动态显微成像系统联用的SM9000
应用案例9/span>
不/span>FKM系统联用研究铜指示植物海州香薶/span>Elsholtzia splendens的叶绿素荧光及其光谱组成'/span>Peng+/span>2013+/span>Environ. Sci. Technol(/span>
不/span>FL3500系统联用研究蓝隐藺/span>Guillardia theta的叶绿素荧光及其光谱组成'/span>Cheregi+/span>2015+/span>Journal of Experimental Botany(/span>
产地9/span>欧洲
参考文献:
1.Bernt G et al. 2017. On the origin of the slow M–T chlorophyll a luorescence decline in cyanobacteria: interplay of short-term light-responses. Photosynthesis Research DOI 10.1007/s11120-017-0458-8
2.Selyanin V et al. 2016. The variability of light-harvesting complexes in aerobic anoxygenic phototrophs. Photosynthesis research 128(1): 35-43
3.Tilstone G et al. 2016. Effect of CO2enrichment on phytoplankton photosynthesis in the North Atlantic sub-tropical gyre. Progress in Oceanography 158: 76-89
4.Mishra K B et al. 2016. Plant phenotyping: a perspective. Indian Journal of Plant Physiology 21(4): 514-527
5.Cheregi O Kotabov E Pr?il O et al. 2015. Presence of state transitions in the cryptophyte algaGuillardia theta. Journal of Experimental Botany 66: 6461-6470
6.Li G Brown C M Jeans J A et al. 2015. The nitrogen costs of photosynthesis in a diatom under current and future pCO2. New Phytologist 205:533-543
7.Kotabov E Jare?ov J Kaa R et al. 2014. Novel type of red-shifted chlorophyll a antenna complex fromChromera velia. I. Physiological relevance and functional connection to photosystems. Biochimica et Biophysica Acta Bioenergetics 1837:734-743
8.?ebela D Olejn?kov J Sotol? R et al. 2014. The slow S to M fluorescence rise in cyanobacteria is due to a state 2 to state 1 transition. BBA 1817: 1237-1247
Peng H et al. 2013. Toxicity and De?ciency of Copper inElsholtzia splendensA?ect Photosynthesis Biophysics Pigments and Metal Accumulation. Environ. Sci. Technol. 47 (12): 6120-6128